肠道可培养共生菌对橘小实蝇不育雄虫生物学特征的影响

橘小实蝇是一种对果、蔬产业造成严重经济损失的害虫，应用昆虫不育技术（SIT）防治橘小实蝇是一种切实可行的科学手段，但辐照会使不育雄虫受到一定程度的损伤。本研究通过在饲料中添加抗生素对肠道灭菌处理来探究肠道可培养共生菌对橘小实蝇生物学及形态学参数的影响。结果表明，抗生素处理组在不育雄虫的蛹重、逆境存活率以及竞争交配力等均显著低于对照组。另外，抗生素处理组和对照组不育雄虫的存活率曲线图表明处理组不育雄虫的寿命时长显著低于对照组，利用Weibull分布拟合模型判断得知两组橘小实蝇存活率曲线均符合基本模型中的Ⅰ型。本研究为进一步利用肠道可培养共生菌来改善橘小实蝇不育雄虫的生理健康提供理论基础。     

亚洲玉米螟核型多角体病毒分离株鉴定及其对草地贪夜蛾的室内毒力测定

本文对从湖北省恩施州亚洲玉米螟幼虫上新分离的一种广谱病毒进行了形态学和分子特征鉴定，并以该病毒与另一种广谱杆状病毒对草地贪夜蛾幼虫进行了生物活性测定。结果表明新分离的核型多角体病毒与薄荷灰夜蛾核型多角体病毒和芹菜夜蛾核型多角体病毒具有较高的同源性，但遗传距离分析不支持它与这两种病毒为同一个种。生物活性测定表明，分离的病毒对草地贪夜蛾3龄幼虫的半数致死剂量是甘蓝夜蛾核型多角体病毒的3.86倍。本研究的结果为开发防治草地贪夜蛾的高效病毒杀虫剂提供了依据。     

Identification of a mitochondrial‐targeting secretory protein from Nocardia seriolae which induces apoptosis in fathead minnow cells

Nocardia seriolae is the main pathogen responsible for fish nocardiosis. A mitochondrial‐targeting secretory protein (MTSP) 3141 with an N‐terminal transit peptide (TP) from N. seriolae was predicted by bioinformatic analysis based on the genomic sequence of the N. seriolae strain ZJ0503. However, the function of the MTSP3141 and its homologs remains totally unknown. In this study, mass spectrometry analysis of the extracellular products from N. seriolae proved that MTSP3141 was a secretory protein, subcellular localization research showed the MTSP3141‐GFP fusion protein co‐localized with mitochondria in fathead minnow (FHM) cells, the TP played an important role in mitochondria targeting, and only the TP located at N‐terminus but not C‐terminus can lead to mitochondria directing. Moreover, quantitative assays of mitochondrial membrane potential (ΔΨm) value, caspase‐3 activity and apoptosis‐related gene (Bcl‐2, Bax, Bad, Bid and p53) mRNA expression suggested that cell apoptosis was induced in FHM cells by the overexpression of both MTSP3141 and MTSP3141ΔTP (with the N‐terminal TP deleted) proteins. Taken together, the results of this study indicated that the MTSP3141 of N. seriolae was a secretory protein, might target mitochondria, induce apoptosis in host cells and function as a virulence factor.     

Isolation and identification of a viral haemorrhagic septicaemia virus (VHSV) isolate from wild largemouth bass Micropterus salmoides in China

Fish rhabdoviruses are a family of viruses responsible for large‐scale fish die‐offs worldwide. Here, we reported the isolation and identification of a member of rhabdoviruses from wild largemouth bass (Micropterus salmoides) in the coastal area of the Pearl River Estuary, China. This virus isolate was identified as viral haemorrhagic septicaemia virus (VHSV) by specific RT‐PCR. Furthermore, the virus (VHSVLB2018) was isolated by cell culture using fathead minnow cells and confirmed by RT‐PCR. Electron microscopy showed the presence of bullet‐shaped viral particles in the cytoplasm of infected cells. The complete sequencing of VHSVLB2018 confirmed that it was genome configuration typical of rhabdoviruses. Phylogenetic analysis based on whole‐genome sequences and G gene nucleotides sequences revealed that VHSVLB2018 was assigned to VHSV genogroup Ⅳa. The pathogenicity of VHSVLB2018 was determined in infection experiments using specific pathogen‐free largemouth bass juveniles. VHSVLB2018‐infected fish showed typical clinical signs of VHSV disease, including darkened skin, petechial haemorrhages and pale enlarged livers, with the cumulative mortalities reached 63.3%–93.3% by 7 days post‐infection. VHSVLB2018 was re‐isolated from dead fish and confirmed by RT‐PCR. Together, this is the first report of isolation and identification of a VHSV isolate from wild largemouth bass in China.     

微咸水膜下滴灌对土壤水盐分布及加工番茄产量的影响

为探明微咸水膜下滴灌对土壤水盐分布及加工番茄生长和产量的影响,通过大田小区试验,设置灌水矿化度和灌水定额两个因素,其中3个灌溉水矿化度水平分别为S1:1 g·L~(-1)、S2:3 g·L~(-1)和S3:5 g·L~(-1),3个灌水定额分别为W1:305 m~3·hm~(-2)、W2:458 m~3·hm~(-2)和W3:611 m~3·hm~(-2),来进一步寻求适宜本地区加工番茄生长的微咸水膜下滴灌灌溉制度。结果表明:覆膜微咸水滴灌条件下土壤含水量垂直方向的变化趋势表现为0～20 cm土层随深度增加含水量逐渐降低、20～100 cm土层随深度增加含水量逐渐增大、60～100 cm范围内土层剖面含水量最大的分布规律;土壤含盐量随着灌水矿化度的增大而增加,且随着灌水量的增加土壤盐分逐渐向水平距滴灌带35 cm处聚集。灌水矿化度超过3 g·L~(-1)时加工番茄株高、茎粗均受到一定程度的抑制作用,但对产量影响不大。本文通过试验得出:灌水定额为611 m~3·hm~(-2)、矿化度为1 g·L~(-1)处理为本地区最佳微咸水膜下滴灌处理,加工番茄生长健壮且产量最高,达到127 613.2 kg·hm~(-2);同时认为,在我国淡水资源比较缺乏的新疆地区可以考虑采用灌水定额458 m~3·hm~(-2)和灌水矿化度3～5 g·L~(-1)的微咸水对盐分中等敏感的加工番茄进行灌溉。     

Effects of different dietary copper sources on the growth and intestinal microbial communities of Pacific white shrimp (Litopenaeus vannamei)

An 8‐week feeding trial was conducted to evaluate the effects of dietary copper sources on growth performance and intestinal microbial communities of juvenile Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous (420 g/kg crude protein) and isolipidic (70 g/kg crude lipid) experimental diets were formulated to contain different copper sources, Diet NSC (no‐supplemented copper), Diet CS (copper sulphate), Diet Availa (copper amino acid complex from Availa^®Cu100) and Diet M (1:1, copper sulphate + copper amino acid complex). A total of 360 Pacific white shrimp juveniles (initial weight: 1.86 ± 0.03 g) were randomly allocated into four groups, with three replicates per group and 30 shrimps each replicate. An Illumina‐based sequencing method was used to analyse the intestinal bacterial composition of shrimp juveniles. The results revealed that shrimps fed Diet M had significantly higher percent weight gain (PWG) than that fed Diet NSC and Diet CS, while there were no significant differences between Diet M and Diet Availa. Survival was not affected by the dietary copper sources. Proteobacteria and Bacteroidetes were dominant in the intestines of Pacific white shrimp. Shrimps fed Diet M and Diet Availa had higher bacterial richness and diversity than those fed Diet NSC and Diet CS with no significant differences among all treatments. Furthermore, Diet M and Diet Availa which contained copper amino acid complex reduced the relative abundance of Vibrionaceae in shrimp intestinal tract. This study indicated that dietary copper sources could affect the intestinal microbial communities and diets supplemented with Availa^®Cu100 could have a positive impact on the growth performance and decrease the potential risk of disease in Pacific white shrimp.     

循环式岩棉栽培水肥一体化装备及控制系统研发

为提高我国循环式岩棉栽培水肥管理水平，自主研发设计了循环式岩棉栽培水肥一体化智能装备及控制系统。系统根据植株的生长发育状态、需水需肥规律以及环境条件，通过智能控制系统全自动地进行营养液的检测、配比和灌溉决策，并结合营养液回收消毒技术，实现营养液的重复利用。系统提供便于操作的硬件设备以及全自动智能运行的控制系统，可对岩棉栽培水肥实现全自动智能精准管理，节水节肥20%以上，实现农业生产的零排放。经过在不同园区的试验和应用，验证该系统运行稳定，适用性强。在宁夏园艺产业园番茄生产应用可达到45.4 kg/m2的高产。      

对高等职业教育中关键能力培养的思考 ——以工程机械运用与维护专业为例

"关键能力"是职业教育领域关注的热点,是一种对劳动者的未来发展起关键作用的能力.文章从关键能力基本内涵出发,在对工程机械制造及销售企业和广泛调研的基础上,论述了工程机械运用与维护专业学生关键能力培养的必要性,并提出在工程机械运用与维护专业课程体系和教学模式中实现学生的职业素质和关键能力培养的措施.     

棉花种质资源收集鉴定与创新利用

十二五期间通过对我国西南地区、南海诸岛、及国外野生棉起源中心实地考察和广泛征集,新收集国内外棉花种质资源1220份,其中陆地棉986份、海岛棉176份、亚洲棉58份,所收集种质均已入国家中期库保存。收集的棉花种质资源分别在河南安阳、海南三亚、新疆阿拉尔进行主要农艺经济性状鉴定和特性鉴定。通过SSR(Simple sequence repeat)分子标记、重测序等技术对收集到的种质进行了分子鉴定,筛选到大量与纤维品质、抗黄萎病、抗旱、抗草甘膦显著关联的基因。在收集保存的种质资源基础上,通过辐射诱变、远缘杂交、复合杂交创造出优质、高产、抗逆等优异种质资源。通过展示向全国科研院所及公司发放棉花种质19 191份次。     

不同规格穴盘育苗对茄苗及其栽培效益的影响

为完善茄子穴盘育苗技术,对比研究了不同规格穴盘育苗的茄苗素质及栽培效益。结果表明:32孔规格穴盘育苗比50孔茄苗素质明显提升;移栽单栋钢架大棚后,32孔穴盘育苗的茄子早熟性比50孔穴盘育苗平均提前5 d,总产量增加15.3%;667 m~2投入成本32孔穴盘育苗比50孔穴盘育苗增加75.3%(35.6元),而收益增加了2 427.9元。因此建议大棚茄子种植采用32孔规格穴盘进行育苗。